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fast digest mspi restriction enzyme  (Thermo Fisher)


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    Thermo Fisher fast digest mspi restriction enzyme
    Fast Digest Mspi Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mspi+fast+digest+restriction+enzyme/fast+digest+mspi+restriction+enzyme/pmc06390101__41598_2019_39591_MOESM1_ESM-20-8-6
    Average 90 stars, based on 1 article reviews
    fast digest mspi restriction enzyme - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Genetic Polymorphisms of Cytochrome P4501A1 (CYP1A1) and Glutathione S-Transferase P1 (GSTP1) and Risk of Hepatocellular Carcinoma Among Chronic Hepatitis C Patients in Egypt.
    Article Snippet: Cytochrome P450 1A1 (CYP1A1) and Glutathione S-transferase P1 (GSTP1) genes are involved in the metabolism of many carcinogens.. Polymorphisms in these genes with altered enzyme activity have been reported.. The present study evaluated the synergistic effect between CYP1A1 and GSTP1 gene polymorphisms and smoking on development of HCV-related liver disease and hepatocellular carcinoma (HCC).

    Mutagenesis:

    Article Title: Genetic Polymorphisms of Cytochrome P4501A1 (CYP1A1) and Glutathione S-Transferase P1 (GSTP1) and Risk of Hepatocellular Carcinoma Among Chronic Hepatitis C Patients in Egypt.
    Article Snippet: Cytochrome P450 1A1 (CYP1A1) and Glutathione S-transferase P1 (GSTP1) genes are involved in the metabolism of many carcinogens.. Polymorphisms in these genes with altered enzyme activity have been reported.. The present study evaluated the synergistic effect between CYP1A1 and GSTP1 gene polymorphisms and smoking on development of HCV-related liver disease and hepatocellular carcinoma (HCC).



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    Image Search Results


    Summary of products and their sizes after digestion with Fermentas Fast Digest® HinfI, Fermentas Fast Digest® MspI and NEB® BanII restriction enzymes

    Journal: BMC Medical Genetics

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T , eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    doi: 10.1186/1471-2350-13-34

    Figure Lengend Snippet: Summary of products and their sizes after digestion with Fermentas Fast Digest® HinfI, Fermentas Fast Digest® MspI and NEB® BanII restriction enzymes

    Article Snippet: In these tubes, the RFLP mixture consisted of 8.5 μL of doubly distilled water, 0.1-0.5 ng of fresh PCR product, 1 X Fermentas Fast Digest® Buffer (Fermentas, Lithuania), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania).

    Techniques:

    Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.

    Journal: BMC Medical Genetics

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T , eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    doi: 10.1186/1471-2350-13-34

    Figure Lengend Snippet: Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.

    Article Snippet: In these tubes, the RFLP mixture consisted of 8.5 μL of doubly distilled water, 0.1-0.5 ng of fresh PCR product, 1 X Fermentas Fast Digest® Buffer (Fermentas, Lithuania), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania).

    Techniques: Agarose Gel Electrophoresis, Multiplex Assay

    Summary of products and their sizes after digestion with Fermentas Fast Digest® HinfI, Fermentas Fast Digest® MspI and NEB® BanII restriction enzymes

    Journal: BMC Medical Genetics

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T , eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    doi: 10.1186/1471-2350-13-34

    Figure Lengend Snippet: Summary of products and their sizes after digestion with Fermentas Fast Digest® HinfI, Fermentas Fast Digest® MspI and NEB® BanII restriction enzymes

    Article Snippet: To identify MTHFR 677 C > T, eNOS +894 G > T and eNOS −786 T > C variants, 1.0 U Fermentas Fast Digest® HinfI restriction enzyme, 1.0 U Fermentas Fast Digest® MspI restriction enzyme and 1.0 U of NEB® BanII restriction enzyme, respectively, were used in separate tubes to avoid errors.

    Techniques:

    Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.

    Journal: BMC Medical Genetics

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T , eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    doi: 10.1186/1471-2350-13-34

    Figure Lengend Snippet: Agarose gel with PCR–RFLP products for analysis of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C . Lane 1 contains Fermentas O’ GeneRuler® Ultra Low Range DNA Ladder (25 bp - 700 bp); Lanes 2, 3 and 4 contain multiplex PCR-RFLP products digested with 1 U of NEB® BanII restriction enzyme (NEB®, England), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania), respectively. The band sizes were 371 bp, 248 bp, 178 bp, 130 bp and 118 bp for all lanes. This indicates that the sample is a TT genotype for MTHFR 677 C > T, GG genotype for eNOS +894 G > T , and TT genotype for eNOS −786 T > C.

    Article Snippet: To identify MTHFR 677 C > T, eNOS +894 G > T and eNOS −786 T > C variants, 1.0 U Fermentas Fast Digest® HinfI restriction enzyme, 1.0 U Fermentas Fast Digest® MspI restriction enzyme and 1.0 U of NEB® BanII restriction enzyme, respectively, were used in separate tubes to avoid errors.

    Techniques: Agarose Gel Electrophoresis, Multiplex Assay